I have a Bio-Rad vertical gel rig that, to my knowledge, hasn’t been successfully incorporated into a research project in 5 years. That is close to $5,000 worth of a pain-in-the-neck gizmo that just doesn’t perform half as well as the vertical rigs I used in Tom Turner’s lab, combined with the shift in methodology that has put SSCP and other fragment analysis protocols way back on our back burner. But I can’t sell it on eBay....here it shall sit, hopefully we can find a use at some point. It has a nice big buffer tank, perhaps I should catch some minnows and make a small aquarium out of it (with temperature control!)?
How many frantic purchases with Applied Biosystems or other companies did I make before realizing that each one of those purchases had a hidden $30-40 shipping cost (dry ice, overnight)?
What about the half-broken chair I inherited in my office? I was told that furniture purchases on my startup funding HAD to be through Chastain’s, a furniture place in downtown Athens. Nothing against that business, but the amount of time I spent bouncing on the 5-6 models available in their showroom, only to leave with a $500 chair charged against my startup, would have been better spend buying three chairs from IKEA on my own credit card. Then I could have had different colors for my changing moods as a young professor.
Most importantly, I would have loved to have had the same amount of money with so few strings attached NOW. Just the few examples of poor expenditures noted above, add those up: I could have a draft transcriptome of any species I want for that amount of money. I could have picked a small group of related species, say the freshwater mussels that we worked on, and dramatically improved our marker availability, understanding of molecular evolution, and so on for just 1/10th of that initial startup package.
But that’s how science rolls. We make mistakes. Technology changes. And we keep writing grant proposals to move on.