I’m always surprised to find that anybody reads this! I got an email about a week ago, though, from a rep at Affymetrix. Presumably they search websites for mention of the Exo-SAP protocol that one of their subsidiaries developed, and which is now sold as a kit under the Exo-SAP-it product line. It is appropriate to note, since this is on the web, that the technique is theirs and should have a little ® after it. We don’t actually use their kit - we buy the ExoI and Antarctic phosphatase from NEB, though I’d be happy to try their product in the future if it gains us a little time or success rate in our work. We are actually still having some hiccups with Sanger sequencing, issues that started about a year ago - some projects sequence beautifully, but most of our mitochondrial work is sequencing poorly unless (and sometimes even when) taxon-specific primers are developed. Another reason to start heading toward nex-gen sequencing solutions!

Tags: lab